P2 dissolvable microcarriers are designed for cell culture processes where efficient cell expansion and simplified harvesting are required. These microcarriers for cell culture are made from a plant-based biopolymer and can be removed through a controlled dissolution step, eliminating the need for filtration or mechanical separation.
P2 supports a range of adherent cell types and can be tailored in stiffness, size, and surface properties depending on process requirements.
P2 dissolvable microcarriers have been evaluated in adherent cell culture using C2C12 myoblasts across both static and bioreactor conditions.
In this study, microcarriers were dissolved using Trypsin or TrypLE to enable recovery of cells without filtration or mechanical separation.
P2 microcarriers are used in microcarrier-based cell culture to support the expansion of adherent cells. The dissolvable format allows removal of the microcarriers without additional separation steps, which can reduce handling and processing time.
The material and surface properties can be adjusted to suit different cell types and applications, including processes where gentle cell recovery is important.
These dissolvable microcarriers are suitable for:
Compared to traditional microcarriers for cell culture, dissolvable systems allow removal of the carrier material without filtration. This can simplify workflows and support more efficient cell recovery, particularly in processes where handling steps need to be minimised.
The development of fast-dissolving, xeno-free microcarriers for cell culture is described in published research:
Development of xeno-free, fast-dissolving microcarriers for scalable stem cell therapy applications
Journal of Cytotherapy (2025)
https://doi.org/10.1016/j.jcyt.2025.03.301
This study reports the development of P2 microcarriers for anchorage-dependent cell culture, demonstrating high cell attachment (~92%), rapid dissolution (as fast as ~3–10 minutes), and efficient cell recovery without mechanical separation.
The results highlight the suitability of dissolvable microcarriers for scalable cell culture, stem cell therapy, and biomanufacturing applications, with tunable properties such as stiffness influencing cell behaviour and productivity.
| Material Composition | Plant-based biopolymer core with xeno-free or animal-derived coatings |
|---|---|
| Material Form | Freeze-dried powder (supplied based on surface area) |
| Stiffness | Soft, Medium, and Hard (customisable: 3-100kPa) |
| Particle Size | 160-260 µm (customisable) |
| Surface Charge | Customisable |
| Dissolvability | Rapid dissolution using TrypLE and our proprietary P2 Dissolving Buffer |
| Sterilisation | Autoclave, heat, or gamma irradiation |
| Grade Availability | Research grade, Food Grade and GMP-compliant options (See Quality Page) |
| Certicate of Analysis | Provided |
