Description
Performance & Process Data
X1 dissolvable microcarriers have been evaluated in adherent cell culture using C2C12 myoblasts across both static and bioreactor conditions.
- Greater than 90% cell attachment within the first 24 hours
- Cell expansion of up to 30-fold over 7 days depending on seeding density
- Cell recovery approaching 100% following enzymatic dissolution
- Post-harvest cell viability exceeding 90%
- Maintains cell differentiation capacity after expansion
Dissolution using TrypLE or Trypsin enables recovery of cells as a single-cell suspension without the need for filtration or mechanical separation.
Microcarriers for Cell Culture Workflows
X1 microcarriers are used in microcarrier-based cell culture where a biologically derived surface is preferred for cell attachment and growth. The gelatin composition supports adhesion across multiple cell types without the need for complex surface modification.
As dissolvable microcarriers, X1 can be removed through enzymatic digestion, allowing recovery of cells without filtration. This approach can reduce processing steps in workflows where enzymatic treatment is already part of the process.
Applications
These dissolvable microcarriers are suitable for:
- Mesenchymal stem cells (MSCs)
- Myoblasts and fibroblasts
- Virus production
- Cell therapy and cultured meat workflows
Why Use Gelatin-Based Dissolvable Microcarriers
Gelatin-based microcarriers for cell culture provide a biologically derived surface that supports attachment of a wide range of adherent cells. X1 is suited to applications where enzymatic harvesting is acceptable and where a familiar extracellular matrix-like environment is beneficial.
Related Publication
The development and performance of dissolvable microcarriers for cell culture is supported by published research on tunable microcarrier systems:
Maximising adherent cell production via customisable and dissolvable bio-polymer microcarriers
Biomedical Materials (2025)
https://doi.org/10.1088/1748-605X/adf1cd
This study evaluates dissolvable microcarriers for adherent cell expansion in bioreactor systems. The microcarriers are tunable in terms of stiffness, size, and surface properties, enabling optimisation across different cell types and bioprocessing conditions.
The study reports improved cell productivity and scalability compared to conventional non-dissolvable microcarriers, highlighting the role of substrate properties such as stiffness in influencing cell growth and yield.
