This whitepaper evaluates the performance of Smart MCs’ X1 dissolvable microcarriers for the expansion of C2C12 mouse myoblast cells in both static and stirred bioreactor culture. The study demonstrates robust cell expansion of up to 30-fold while maintaining myogenic differentiation, highlighting the suitability of X1 microcarriers for scalable muscle cell culture. Download the full whitepaper for the complete methodology, experimental data, and analysis.
Figure 1. Representative images of C2C12 cells cultured on Smart MCs’ X1 dissolvable microcarriers throughout the study. (A) Brightfield microscopy showing C2C12 cells attached to X1 microcarriers. (B) Scanning electron microscopy (SEM) image showing cell attachment to the microcarrier surface. (C) Immunofluorescence image of differentiated C2C12 cells stained for myosin heavy chain (Myh1, red) and nuclei (DAPI, blue).
