Pathogen Purification Using Inertial Microfluidics

Microfluidic Systems for Size-Based Pathogen and Sample Purification

 

Smart MCs provides microfluidic systems for pathogen purification based on inertial microfluidics. These devices enable size-based separation of microorganisms, cells, and debris from biological samples under continuous flow conditions.

The system is designed to support purification of complex samples from human, animal, and environmental sources, reducing unwanted components such as debris and background cells.

 

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Description

Why Use Microfluidic Pathogen Purification?

  • Label-Free Separation – No antibodies or reagents required
  • Size-Based Sorting – Separation of pathogens, cells, and debris by size
  • Inertial Microfluidic Operation – Passive focusing in spiral microchannels
  • High-Throughput Processing – Suitable for large sample volumes
  • Low-Shear Conditions – Designed to minimise mechanical stress on cells and microorganisms

Applications

  • Pathogen Isolation – Enrichment of bacteria and parasites from biological samples
  • Sample Purification – Removal of debris and unwanted cells from complex fluids
  • Clinical and Research Samples – Processing of blood, tissue, and environmental samples
  • Cell Subpopulation Separation – Isolation of specific cell groups from mixed samples
  • Genomics and Proteomics – Sample preparation for downstream molecular analysis
  • Environmental Microbiology – Processing samples for microorganism detection

Related Publication

The pathogen purification approach is supported by published research demonstrating size-based separation of microorganisms using inertial microfluidics:

Giardia purification from fecal samples using rigid spiral inertial microfluidics
Biomicrofluidics (2022)
https://doi.org/10.1063/5.0069406

This study demonstrates the use of a rigid spiral inertial microfluidic device for purification of Giardia cysts from fecal samples. The system enables separation of pathogens from highly turbid samples, achieving recovery rates up to ~75% at a throughput of ~0.75 mL/min.

The results show effective removal of surrounding debris, allowing visual identification of purified cysts after a single pass. The approach is scalable and can be extended to purification of other gastrointestinal pathogens of similar size.

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Additional information

Product Type

Microfluidic pathogen purification device

Technology

Inertial microfluidics (spiral microchannels)

Separation Method

Label-free, size-based separation

Size Range

Application-dependent (micron-scale particles and cells)

Processing Mode

Continuous flow

Material

PDMS

Sterility

Autoclave and gamma irradiation available upon request

Applications

Pathogen isolation, sample purification, biological processing

Documentation

Certificate of Conformity (CoC) available upon request

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